Association between consumption of cruciferous vegetables and condiments and excretion in urine of isothiocyanate mercapturic acids

Auteur(s) :
Vaes WHJ., Van Den Berg R., Vermeulen M., Freidig AP., Van Bladeren PJ.
Date :
Juin, 2006
Source(s) :
Journal of agricultural and food chemistry. #54:15 p5350-5358
Adresse :
Addresses: Vaes WHJ (reprint author), TNO Qual Life, Business Unit Analyt Res, POB 360, NL-3700 AJ Zeist Netherlands TNO Qual Life, Business Unit Analyt Res, NL-3700 AJ Zeist Netherlands E-mail Addresses: wouter.vaes@tno.nl Publisher: AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, http://pubs.acs.org Discipline: AGRICULTURAL CHEMISTRY CHEMISTRY & ANALYSIS

Sommaire de l'article

Abstract: A high intake of cruciferous vegetables is associated with a reduced risk of cancer and cardiovascular diseases. This protective effect has been linked to isothiocyanates, enzymatic hydrolysis products of glucosinolates. In this study, the metabolic fate of glucosinolates and isothiocyanates after ingestion of 19 different cruciferous vegetables was studied in three male subjects. After the consumption of 13 cruciferous vegetables ( glucosinolate content, 0.01-0.94 mmol/kg) and six condiments ( isothiocyanate content, 0.06-49.3 mmol/kg), eight different isothiocyanate mercapturic acids were determined in urine samples. Excretion levels after the consumption of raw vegetables and condiments were higher (bioavailability, 8.2-113%) as compared to cooked vegetables (bioavailability, 1.8-43%), but the excretion rate was similar (t(1/2) = 2.1-3.9 h). Isothiocyanates in urine remain longer at a nonzero level after the consumption of glucosinolates from cooked vegetables, as compared to raw vegetables and condiments, and maximal levels in urine were reached about 4 h later. Isothiocyanate mercapturic acids can be used as a biomarker to reflect the active dose of isothiocyanates absorbed.
Author Keywords: cruciferous vegetables; glucosinolates; HPLC-MS analysis; kinetics; biomarker
KeyWords Plus: BENZYL ISOTHIOCYANATE; BRASSICA VEGETABLES; QUANTITATIVE-DETERMINATION; CYSTEINE CONJUGATE; CANCER PREVENTION; METABOLISM; HUMANS; BIOMARKER; FRUIT; IDENTIFICATION

Source : Pubmed
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