Direct correlation of glutathione and ascorbate and their dependence on age and season in human lymphocytes

Auteur(s) :
Payette H., Cantin AM., Fulop T., Lenton KJ., Therriault H., Wagner JR.
Date :
Mai, 2000
Source(s) :
AMERICAN JOURNAL OF CLINICAL NUTRITION. #71:5 p1194-1200
Adresse :
"WAGNER JR,UNIV SHERBROOKE,INST GERIATRIE CTR RECH;PAVILLON YOUVILLE 1036 RUE BELVEDERE SUD;SHERBROOKE,PQ J1H 4C4 CANADA.rwagner@courrier.usherb.ca "

Sommaire de l'article

Background: Endogenous reactive oxygen species appear to contribute to aging and cancer and dietary antioxidants, present in fruit and vegetables, counteract these effects.Objective: The objective was to examine the association between intracellular glutathione, ascorbate (vitamin C), and oc-tocopherol (vitamin E) in human lymphocytes.Design: The study group consisted of 240 healthy nonsmoking volunteers with an approximately equal number of male and female subjects subdivided into 3 age groups: 18-39, 40-59, and greater than or equal to 60 y). Glutathione, glutathione disulfide, ascorbate, and alpha-tocopherol were measured in lymphocytes by HPLC.Results: The average concentration of antioxidants in lymphocytes was 27 +/- 8 nmol/mg protein for glutathione, 21 +/- 8 nmol/mg protein for ascorbate, and 0.4 +/- 0.2 nmol/mg protein for alpha-tocopherol. There was a strong positive correlation between glutathione and ascorbate (r = 0.62, P < 0.001). No correlation was observed for glutathione and ascorbate with alpha-tocopherol. The concentration of glutathione in lymphocytes was inversely correlated with age (r = -0.19, P < 0.01), as was that of ascorbate (r = -0.22, P < 0.01), with 10-20% lower values in elderly than in young and elderly subjects. The concentrations of glutathione in lymphocytes were as much as 25% higher and those of ascorbate were as much as 38% higher during the summer than during the winter. The seasonal variation of ascorbate in lymphocytes was described by a linear function for age and a periodic sine function for season.Conclusion: Glutathione and ascorbate are directly correlated in human lymphocytes.

Source : Pubmed
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