Inhibition of cell proliferation and in vitro markers of angiogenesis by indole-3-carbinol, a major indole metabolite present in cruciferous vegetables

Auteur(s) :
Chen YH., Wu HT., Linder SH.
Date :
Juin, 2005
Source(s) :
Journal of agricultural and food chemistry. #53:13 p5164-5169
Adresse :
Reprints: CHEN YH,TAIPEI MED UNIV,SCH NUTR & HLTH SCI;250 WU HSING ST; TAIPEI 110, TAIWAN. Research Institutions: Taipei Med Univ, Sch Nutr & Hlth Sci, Taipei 110, Taiwan. Discipline: AGRICULTURAL CHEMISTRY CHEMISTRY & ANALYSIS

Sommaire de l'article

A variety of studies have suggested a cancer protective role of cruciferous vegetables. In the present study, we investigated the effect of indole-3-carbinol (I3C), a major indole metabolite in cruciferous vegetables, on cell proliferation and in vitro markers of angiogenesis in phorbol myristate acetate (PMA)-stimulated enclothelial EA hy926 cells. The results showed that I3C inhibited the growth of EA hy926 cells in a concentration-dependent manner. The capillary-like tube formation by PMA-activated enclothelial cells was significantly suppressed by I3C, and such inhibition was associated with decreased vascular enclothelial growth factor (VEGF) and increased interleukin-8 (IL-8) secretion, but not with the expression of VEGF receptor-2 protein. Additionally, gelatin zymography analysis indicated that I3C suppressed activities of matrix metal loproteinases-2 (MMP-2) and MMP-9 stimulated by PMA. These results suggest that the dietary I3C may be useful in the treatment of human cancers and angiogenic diseases.

Source : Pubmed