Separation and determination of flavonoids and other phenolic compounds in cranberry juice by high-performance liquid chromatography

Auteur(s) :
Cheng CH., Deng YW., Zuo YG.
Date :
Avr, 2001
Source(s) :
JOURNAL OF CHROMATOGRAPHY A. #913:1-2 p387-395
Adresse :
"ZUO YG,SE MASSACHUSETTS UNIV,DEPT CHEM & BIOCHEM; N DARTMOUTH MA 02747, USA.yzuo@umassd.edu "

Sommaire de l'article

A HPLC method was developed for the separation and determination of flavonoid and phenolic antioxidants in cranberry juices. Free flavonoid and phenolic compounds were fractionated into neutral and acidic groups by means of a solid-phase extraction method, followed by subsequent HPLC separations. Combined flavonoids and phenolics were hydrolyzed by acid before HPLC analysis. This developed method provides a fast and high resolution of individual flavonoid and phenolic compounds. In cranberry fruit, flavonoids and phenolic acids exist predominantly in combined forms, such as glycosides and esters. A total of 400 mg of total flavonoids and phenolic compounds/l of sample was found in a freshly squeezed cranberry juice, which was distributed as about 44% of phenolic acids and 56% of flavonoids. Benzoic acid was the major phenolic compound. Major flavonoids in the freshly squeezed cranberry juice were quercetin and myricetin.

Source : Pubmed
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