Antiinflammatory properties of the muscadine grape (vitis rotundifolia)

Auteur(s) :
Greenspan P., Bauer JD., Pollock SH., Gangemi JD., Mayer EP., Ghaffar A., Hargrove JL., Hartle DK.
Date :
Nov, 2005
Source(s) :
Journal of agricultural and food chemistry. #53:22 p8481-8484
Adresse :
Univ Georgia, Nutraceut Res Labs, Athens, GA 30602 USA Univ Georgia, Nutraceut Res Labs, Athens, GA 30602 USA Mercer Univ, So Sch Pharm, Athens, GA 30341 USA Clemson Univ, Inst Nutraceut Res, Clemson, SC 29634 USA Univ S Carolina, Dept Pathol & Microbiol, Columbia, SC 29206 USA E-mail Addresses:

Sommaire de l'article

The muscadine grape possesses one of the highest antioxidant levels among fruits; yet, the effect of this fruit on mammalian metabolic systems has not received significant attention. To examine the antiinflammatory properties of the muscadine, grape skins were dried, pulverized, and extracted (10% w/v) with 50% ethanol. The extract was then tested in two different assays: the release of superoxide in phorbol myristate acetate-activated neutrophils and the release of cytokines [tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-beta), and interleukin-6 (IL-6)] by lipopolysaccharide-activated peripheral blood mononuclear cells. The release of superoxide and cytokines was inhibited by increasing concentrations of the extract. A 1:100 dilution of the extract inhibited superoxide release by approximately 60% while the release of TNF-alpha and IL-1 beta was reduced at a dilution of 1:200 by approximately 15 and 90%, respectively (all P < 0.05). The inhibition pattern on the release of IL-6 was similar to that seen with TNF-a. In a related in vivo study, rats were fed a diet containing 5% (wt/wt) dried muscadine grape skins for 14 days and then were injected with carrageenan in the foot pad. After 3 h, paw edema was measured and the rats on the grape skin diet had approximately 50% less paw edema than controls (P < 0.05). These results demonstrate that the muscadine grape skin powder possesses significant in vitro and in vivo anti inflammatory properties.

Source : Pubmed